show Abstracthide AbstractThe status of the South-American Histiotus bat species is controversial and their phylogenetic relationships have been so far unresolved. Our study shows that Histiotus have experienced a radiation after the relatively recent colonization to the Neotropics, with evidence of their speciation being still incomplete. This is the first study to investigate the systematic relationships between Histiotus bats at a whole-genome level and more importantly, highlights importance of inspecting both mitochondrial and nuclear DNA evidence in understanding the evolutionary history of species.